{"created":"2023-05-15T12:06:30.915197+00:00","id":27883,"links":{},"metadata":{"_buckets":{"deposit":"eaacc1be-1055-4156-9192-c8255e947b4e"},"_deposit":{"created_by":5,"id":"27883","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"27883"},"status":"published"},"_oai":{"id":"oai:naruto.repo.nii.ac.jp:00027883","sets":["1846:1902:1903","1846:1904:2222:2249:2316:2379"]},"author_link":["23918","23919","23920","23921"],"item_24_biblio_info_10":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-14","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"339","bibliographicPageStart":"332","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"鳴門教育大学研究紀要","bibliographic_titleLang":"ja"},{"bibliographic_title":"Research bulletin of Naruto University of Education","bibliographic_titleLang":"en"}]}]},"item_24_description_8":{"attribute_name":"抄録（英）","attribute_value_mlt":[{"subitem_description":"The scanning electron microscopy（SEM）has already been exploited in the medical and biological fields of research. Since SEM observation requires to place specimen in a vacuum chamber, it is impossible to observe the wet biological specimens like planktons as they are. Furthermore, it is required for the insulating specimens to be subjected to pretreatments such as coating of metal and carbon by vapor deposition or sputtering in order to prevent from charging the specimens. Thus, the pretreatment of the biological specimens is required for SEM observation. The conventional method generally consists of fixation, dehydration, freeze−drying, and Au sputtering. However, this method takes a lot of times and steps. Recently it is reported that the SEM observation of biological specimens can be simple by the pretreatment with room temperature ionic liquid（RTIL）. RTILs are liquid salts even at or below room temperature, and have several attractive features such as negligible vapor pressure and high ionic conductivity. The pretreatment with RTIL consists of immersing the specimens in the RTIL solution or spraying the RTIL solution onto the specimens, and subsequently blowing off the excess ionic liquids. In the present study, the pretreatment with RTIL is applied to the SEM observation of planktons as a typical sample of the wet biological species, and the optimum protocol is investigated. Ionic liquid of 1－ Ethyl－3－methylimidazolium methylphosphonate（EMI−BF4 : KantoChemical Co., Inc.,）is used in the experiment. This ionic liquid is diluted by ethanol. The clear SEM image of Artemia saline as a plankton is successfully obtained by using the pretreatment with the ionic liquid. It is found that there is the optimum concentration of the ionic liquid［1％（w/w）］for the SEM observation of planktons.","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_24_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24727/00027877","subitem_identifier_reg_type":"JaLC"}]},"item_24_publisher_11":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"鳴門教育大学","subitem_publisher_language":"ja"}]},"item_24_publisher_12":{"attribute_name":"出版者(別表記)","attribute_value_mlt":[{"subitem_publisher":"Naruto University of Education","subitem_publisher_language":"en"}]},"item_24_source_id_13":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1880-7194","subitem_source_identifier_type":"EISSN"}]},"item_24_source_id_14":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12124651","subitem_source_identifier_type":"NCID"}]},"item_24_text_29":{"attribute_name":"出版物サブタイトル","attribute_value_mlt":[{"subitem_text_value":"自然科学編"}]},"item_24_text_30":{"attribute_name":"出版物サブタイトル(別表記)","attribute_value_mlt":[{"subitem_text_value":"Natural Sciences"}]},"item_24_text_31":{"attribute_name":"本文の有無","attribute_value_mlt":[{"subitem_text_value":"本文あり"}]},"item_24_text_5":{"attribute_name":"所属","attribute_value_mlt":[{"subitem_text_value":"鳴門教育大学生活・健康系コース（技術・工業・情報）"},{"subitem_text_value":"鳴門教育大学院学校教育研究科生活健康系コース（技術・工業・情報）"}]},"item_24_text_6":{"attribute_name":"所属（英）","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Naruto University of Education"},{"subitem_text_language":"en","subitem_text_value":"Graduate School of Education, Naruto University of Education"}]},"item_24_version_type_23":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"宮本, 賢治","creatorNameLang":"ja"},{"creatorName":"ミヤモト, ケンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山下, 泰史","creatorNameLang":"ja"},{"creatorName":"ヤマシタ, タイジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"MIYAMOTO, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"YAMASHITA, Taiji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-03"}],"displaytype":"detail","filename":"KK29028.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KK29028.pdf","url":"https://naruto.repo.nii.ac.jp/record/27883/files/KK29028.pdf"},"version_id":"d7451e2a-233a-4c70-a4c5-257ffab14b01"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"イオン液体","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"走査型電子顕微鏡","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"水生微生物","subitem_subject_language":"ja","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"イオン液体を用いた水生微生物の電子顕微鏡観察の方法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イオン液体を用いた水生微生物の電子顕微鏡観察の方法","subitem_title_language":"ja"},{"subitem_title":"Pretreatment of plankton with room temperature ionic liquid for SEM Observation","subitem_title_language":"en"}]},"item_type_id":"24","owner":"5","path":["1903","2379"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-09-03"},"publish_date":"2018-09-03","publish_status":"0","recid":"27883","relation_version_is_last":true,"title":["イオン液体を用いた水生微生物の電子顕微鏡観察の方法"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2023-12-14T08:17:35.288353+00:00"}